Used to store soil fungi.
Approx 5g of soil is mixed with 1L of water. The water is shaken thoroughly, and filtered. Agar is added, the mixture autoclaved and plates poured.
Used to grow dung fungi because of the fatty acids in dung.
Approx 3 pellets of fresh rabbit dung or equivalent is placed in 100ml water and blended. 2% agar is added, the mixture autoclaved and plates poured.
A good generalist medium.
|Water make up to 1litre|
Dice potatoes and boil for one hour. Sieve to remove coarse fragments, add dextrose and agar, add water to make 1L, autoclave, mix and pour.
Used to grow acidic fungi.
Mix, adjust pH with CaCO4, autoclave and pour plates.
A good generalist medium, to which a variety of ingredients may be added. Note that MA is commonly acidic.
Malic acid (0.5% for basidiomycetes), Peptone (0.1% for zygomycetes), sucrose (20% for high osmotic pressure), Czapek salts (for extra minerals).
Dissolve malt extract, add other ingredients and agar, autoclave and pour plates.
Good generalist medium which is clear.
|Sucrose (glucose can be used)||20g|
If you make up stock soltions, keep the phospahte separated, add to the final solution. Mix, autoclave and pour plates.
For culture of dermatophytes.
Dissolve and mix. Adjust to pH 5.6, autoclave and pour plates.